Determination of Chinese herbal medicine

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Determination of content

Chromatographic conditions and system suitability test octadecylsilane bonded silica as a filler; acetonitrile-water (56:44) as the mobile phase; detection wavelength of 235nm. The number of theoretical plates should not be less than 2,000 as calculated by the peak of Uglyol ether lactone.

Accurately weigh the reference solution of the preparation of the reference substance of linum lindenii 10 mg, place it in a 100-ml volumetric flask, dissolve it with methanol and dilute it to the mark, shake it up, accurately measure 10 ml, set it into a 25-ml volumetric flask, and add methanol to the mark. Shake well and obtain (40 μg of Uglyol ether lactone per 1 ml).

Preparation of the test solution to take this product coarse powder about 1g (while taking this product coarse powder to determine moisture (Appendix IX H second method)), accurately weighed, set Soxhlet extractor, add ether 50ml, extract 4 Hours, the extract was evaporated, the residue was dissolved in methanol in portions, transferred to a 50 ml volumetric flask, added to the mark with methanol, shaken, filtered, and the filtrate was taken.

The assay accurately draws 10 μl of each of the reference solution and the test solution, and injects it into the liquid chromatograph for determination.

This product is calculated according to the dry product, containing lindione ether lactone (C15H16O4) shall not be less than 0.030%.

乌药


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