Preparation and precautions for MH medium Professional biochemical reagent supplier Guduo Bio provides you >>> The MH medium is the mh broth medium, and the MH medium refers to the hydrolyzed casein (Mueller-Hinton) medium, which is the aerobic and facultative anaerobic susceptibility used by the NCCLS National Clinical Laboratory Standards Committee. The test standard medium, pH 7.2 ~ 7.4, agar thickness of 4mm. This article mainly introduces the preparation and precautions of MH medium. The principle of MH medium: Beef meal and acid hydrolyzed casein provide nitrogen sources, vitamins and amino acids; soluble starch absorbs toxic metabolites; agar is a coagulant for the medium. Preparation of MH medium: 1, 3.8g M-H agar base + 100mL distilled water 2, autoclaving (15 lbs, 121 ° C) 20min 3. Cool the plate to 50 ° C and pour the plate to a thickness of 4 mm. MH medium notes: 1. It is generally not recommended to culture in an environment where the concentration of CO2 is high when cultivating bacteria, because CO2 will affect the pH of the medium. 2. If it is necessary to culture with CO2, it should include a test plate that measures the effect of CO2. 3. Sugars should not be used as an additive to MH agar because they affect the growth rate of the bacteria and the pH of the medium. 4. The addition of lysed horse blood to the medium further reduces the level of thymidine nucleosides, which can always be the growth of thymidine-dependent nucleoside-dependent bacteria. YELLOW COLOR VACUMMN FRESH WAXY CORN FOR HUMAN EATTING , SWEET CORN PLANTED IN INNER MONGOLIA CHINA. VACUMMN FRESH WAXY CORN FOR HUMAN EATTING Wuyuan county dafeng oil food co.,ltd , https://www.dafengfood.com.cn