The non-nonsense is not to say, we should first read the topic: The microtubule-associated protein PRC1 promotes early recurrence of hepatocellular carcinoma in association with the Wnt/β-catenin signalling pathway

Very classic: A gene plays a D- function in the C disease through the B- signal pathway. It is recommended that the majority of doctors and friends who study the gene function to write a tender or write an article should follow this method as clearly as possible, covering all the points of the article.

The author did not know where to find such a gene PRC1, and found that the mRNA and protein expression levels of this gene gradually increased in adjacent tissues, in situ and recurrent foci, and also correlated with prognosis of patients, and the TCGA database was used to assist the proof. The evidence is solid. (Dr.S Tips: When many friends write articles, they think that they must write out the process of finding the gene to find out the gene. I am afraid that the reviewer will ask how the gene comes from. In fact, there is no need to worry about it. It is not very important to find, it is important that the genes have clinical relevance and function)

Since the recurrence of genes and liver cancer is so relevant, is it functional? The following eyes can also know that the authors should use cell and animal models to verify gene function, and found that after the gene was knocked down, the cells did not increase in value and did not invade and metastasize. Subcutaneous tumors are not long, beautiful data. (Dr.S Tip: If you have done a lot of similar cell function experiments, then the cell line is ok with two strains. If you do not have a lot of cell function experiments, consider doing more cells).

Clinical relevance and in vitro and in vivo functions have been verified. What should I do next? An article that wants to score 10 points is definitely going to be an in-depth study. The authors found that the promoter region of PRC1 has a TCF4 binding site and therefore may be affected by the WNT pathway. The experimental results confirm the author's conjecture, and the PRC1 gene can also form a protein complex with Axin1/APC/GSK3B and participate in the regulation of downstream target genes of the WNT pathway. A feedback negative feedback regulation path is formed.

Well, another beautiful story was born. WNT3a activates the WNT pathway by binding to extracellular receptors, which leads to an increase in the expression of PRC1. In addition, excess PRC1 protects B-catenin from degradation by inhibiting the formation of APC complexes. The targeted oncogenes downstream of the WNT pathway (including PRC1 itself) are activated to form a vicious circle, which ultimately leads to the occurrence of liver cancer and promotes the recurrence of liver cancer.

Interpretation of Dr.S:

1. To send Hepatology, you must first have a clinically relevant and functional gene that has not been reported in the disease under investigation.

2. If you want to rush an article with more than 10 points, the mechanism part can be pulled upstream and then pulled down. It must explain which proteins can combine with the target gene to form a complex, or which base in the promoter region of the target gene. The base is regulated by the upstream protein, and the direct interaction mechanism is the best.

3. It is best to find the star gene or pathway in the mechanism part, so it is easier to make the function recovery experiment, because the function of the downstream gene is definitely strong.

4. High-scoring articles can use several cells to verify the function (human sea tactics are very effective many times), and only one cell can be used as a mechanism.

According to the experience of the Jikai gene, there are many candidate genes screened by high-throughput methods such as chip, sequencing, and mass spectrometry from the sample, but the probability of obtaining a new functional gene is only about 10%, so you want to screen and verify. It is very difficult to get a new functional gene. After three years, Jikai Gene has spent hundreds of millions of dollars and has completed the functional screening of nearly 6000 tumor-related genes. It has found a number of clear tumor function marker genes, waiting for you to hunt for treasure~~~

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