Developed and manufactured by Diagenode, Belgium, the Bioruptor non-contact fully automatic ultrasonic breaker is an unparalleled quality, efficiency and safety of traditional ultrasonic methods. This innovation has been widely used in Europe and the United States, cited by hundreds of authoritative magazines, currently the best-selling two, Bioruptor Plus and Bioruptor Pico. Bioruptor Plus is more versatile. Bioruptor Pico is more focused on processing small amounts of samples. It is mainly used for ChIP experiments and pre-processing of second-generation sequencing. Bioruptor sonicator is becoming an indispensable standard for ChIP (chromatin immunoprecipitation) research platform. tool. How Bioruptor works During the experiment, the Bioruptor ultrasonic sampler was placed in a separate, fully sealed centrifuge tube. There was no cross-contamination between the samples, which avoided the propagation of aerosol and enhanced the biosafety of the experiment. For 15ml and 50ml centrifuge tube adapters, the Bioruptor sonicator also features a patented resonance system that conducts ultrasonic waves through a metal rod inserted into the centrifuge tube that is in direct contact with the sample. This metal rod can reflect the ultrasonic waves generated by the ultrasonic generating device at the bottom of the water tank, and enhance the ultrasonic breaking effect of the sample. However, unlike the conventional probe, the metal rod does not directly generate ultrasonic waves and does not cause corrosion and the like. Bioruptor usage precautions For the requirements of water, we must first clean it. Simply put, there is no impurity. At this time, many students will think of using ultrapure water directly, which is absolutely clean enough. Unfortunately, the ultra-pure water is deionized water. The various elemental ions that should have been removed from the water have been removed. The microscopic explosive force mentioned above has been greatly reduced, so it is necessary to use clean non-deionized water. . The water in the machine sink and the water in the sample tube must follow this principle! Off-topic, why is ultrapure water not drinkable, because the ultra-pure water itself has insufficient ion density, and naturally it will precipitate trace elements in the human body for ion balance. By the same token, if the Bioruptor often uses deionized water, the sink gradually forms a rough black spot from the smooth stainless steel surface, because the deionized water precipitates the metal ions in the sink. As a result, the experiment was not satisfactory and the Bioruptor's sink was also damaged. The Bioruptor tank recommends that you use pure water or single distilled water. The sample in the sample tube is ready for use with conventional buffers. The second is to avoid the high temperature of the Bioruptor. Bioruptor's consumables selection First, the choice of imported domestically mixed, it is more difficult to choose, and the stability of a brand's batch is not good, the ultrasound needs to penetrate the pipe wall, which requires the uniformity of the pipe wall is better. After all, it is not a common consumable, the amount is small, simply choose to import, such as the commonly used Axygen, Eppendorf, 15ml or 50ml can also choose Corning and Falcon. For precious samples, low-adsorption sample tubes can be used. There are several major brands imported. Bioruptor manufacturers are low-adsorption. For some experiments, they are demanding and very difficult to handle. Bioruptor tips 2. Fill up the adapter every time 3. The three-point method oscillates the centrifuge tube. Especially for the system with very small sample volume, such as 10ul, the Bioruptor has high ultrasonic energy. It is easy to put a small amount of sample in the sample tube to the tube wall and the ultrasonic failure. Therefore, periodically centrifuge the sample tube according to your experimental cycle number to ensure that the sample is effectively ultrasonic. For example, if you want to do 15 cycles, adjust the machine to 5 cycles. After 5 cycles, the stepper motor will stop rotating. Take out the adapter, take out the sample tube and shake it, then machine it for 5 cycles. The adapter is also taken out, and the sample tube is taken out and shaken, and then processed for 5 cycles. At the same time, it is also recommended not to establish too small sample system, try to be more, so that you can avoid invalid ultrasound. We're professional Neuro-Endoscopy Instruments
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The Bioruptor Ultrasonic Crusher uses a design that installs an ultrasonic generator at the bottom of the sink. The traditional probe sonicator, the microfluidic phenomenon caused by ultrasound can only appear in the area close to the probe, while the Bioruptor sonicator is installed with ultrasonic waves at the bottom of the sink. The water is completely in the range of the ultrasonic wave, and the ultrasonic effect is widely distributed and balanced, which reduces the formation of foam. During the experiment, the Bioruptor Ultrasonic Crusher automatically rotates the centrifuge tube to make the ultrasonic energy distribution more uniform. 
Bioruptor schematic
The first is water.
Water is divided into machine water and laboratory water. The Bioruptor Sink Non-Contact Ultrasonic Crusher, as its name implies, is the ultrasonic energy that passes through the sample tube in the liquid phase through the water.
The physical principle that ultrasonic waves can be broken is quite complicated. At present, there is no detailed description. The industry basically agrees that it is Cavitation. Most of the translations are cavitation effects. Under high frequency pulse slap, liquid will produce countless high voltage points. And the low pressure point, the so-called voids are formed, and the numerous voids are only kept in milliseconds and are exploded by the continuously formed voids. In this process, a super microscopic explosive force is formed in the liquid, and this force can break the cells. Degrading proteins can further cut nucleic acids and become shorter and shorter.
The Bioruptor ultrasonic generator is glued under the water tank. Under high temperature conditions, the ultrasonic generator itself is damaged. Secondly, the firmness of the ultrasonic generator and the bottom of the water tank is damaged. It is known that the Bioruptor bump vibration should be avoided, and it should not be centrifuged. Machines, shakers, etc., which generate vibration when working, are placed on a test bench. It is best to find a good room for the Bioruptor alone, and place it on a solid countertop. Also, don't move around. In short, Bioruptor is afraid of heat and vibration. Keep this in mind.
What can Bioruptor do?
The Bioruptor is quite versatile, and you can't do it if you can't think of it. Compared with the traditional probe type ultrasonic breaker, it is more widely used. The limitation is the volume of a single sample, which can be small and not too large.
Animal and plant tissue, broken plant and animal cell, broken yeast, fungi, broken bacteria, tubercle bacillus, bacteria, etc., extraction, purification, purification of nucleic acids, proteins and various metabolic compounds, preparation of nuclear chromosomal chromatin, fragmentation, nucleic acid fragmentation, adenovirus release, high-cost recombinant adenovirus Extraction of viral DNA
Soil sample crushing preparation of nanoparticles to dissolve various insoluble reagents to prepare oil-water emulsion ellipsis
Daily maintenance of Bioruptor
1. Ensure that the water temperature in the sink is stable at 4 ° C
The main thing is to avoid overheating of the Bioruptor, while the water density at 4 °C is the highest and the ultrasound effect is also the best. The Bioruptor ultrasonic generator is glued under the water tank to drive the water tank to generate high-frequency ultrasonic waves, part of which acts on the sample, and the other part is basically converted into heat energy. The Bioruptor is powerful and the heat is very fast. For the Bioruptor, it is most afraid of heat. Heat is afraid of heat and extremely damages the life of the instrument.
2. Ensure that the Bioruptor is used for less than one hour. After use, ensure that the instrument is not in operation for at least half an hour. The water temperature of the sink is guaranteed to be 4 °C. The core component below the Bioruptor sink is not 4 °C, so it must be After working for one hour at the Bioruptor, ensure that the Bioruptor host is inactive for at least half an hour, and the host heatsink can effectively cool down.
3 guarantee to replace the pure water once a week
The water in the Bioruptor sink should be replaced regularly to ensure the cleanliness of the water. If it is not used for a long time, please drain it. Again, it is forbidden to use deionized water
4. Ensure that the Bioruptor stops the ultrasound and then remove the sample tube adapter. The sample tube adapter is placed above the Bioruptor sink and meshes with the stepper motor gear on the sink cover. When the Bioruptor is working, the motor rotates at a constant speed and drives the sample tube adapter to rotate. The energy distribution is more uniform. After the set number of cycles is completed or the stop button is pressed, the motor will stop rotating. The adapter cannot be taken out directly in the middle. Such improper operation will damage the stepper motor for a long time.
5. Ensure that the ultrasonic time of each cycle is less than or equal to the pause time. The reason is very simple, cool down and cool down, fearing that heat is afraid of heat and heat. The Bioruptor defaults to 30sON/30sOFF. You can also follow the default.
The second is to choose a tube with high transparency and thin wall, which is relatively hard and brittle, which facilitates the transmission of ultrasonic waves and improves the efficiency of ultrasonic utilization.
Third, once a pipe is selected, do not replace other pipes in the middle of the experiment.
It is recommended to buy as much as possible according to the experimental consumption, and try to ensure that there is no error in the consumables.
In addition, some laboratories will use the Bioruptor plus non-contact automatic ultrasonic breaker to break down micro-samples, such as microbial and animal and plant tissues, because the ultrasonic intensity of the instrument is very large, even if It is not very good to control the degree of breakage in the low-end equipment, it is likely to destroy the target product. In order to reduce the ultrasonic efficiency, it is necessary to select the tube in the opposite way to the second principle.
1. The first Bioruptor will use the highest efficiency every day. The Bioruptor sonicator also has the ultrasonic attenuation. The ultrasonic generator will have fatigue attenuation. The reason is nothing more than the ultrasonic generator becomes hot after work, so choose The first time you use Bioruptor every day, the efficiency is also the best.
This is where the Bioruptor is superior to the traditional probe-type ultrasonic breaker. The traditional ultrasonic breaker can only process one sample at a time. The experimental cycle is long. The Bioruptor sonicator can detect 6 to 12 samples at a time. The experiment efficiency is high. There are batch-to-batch differences.
The Bioruptor Ultrasonic Crusher manufacturer recommends that the spare sample tube holes be filled with blank control sample tubes every time you process several samples, ensuring that each ultrasonic energy fraction is uniform and easy to establish stable experimental optimization conditions. Processing several samples can guarantee that the same processing results will be obtained each time with the same time.
4. Finally, it is recommended that everyone is a principle. If the ultrasound time is less, then the ultrasound time is not longer. The effect is better. This also follows the marginal effect. If the target product is obtained through 5 ultrasonic cycles, the 6 cycles are not obvious. The improvement will be guaranteed in the future with only 5 ultrasound cycles. Ultrasonic waves do not selectively break the sample, and the longer the processing time, the more the interference term is caused. Therefore, I would like to advise everyone that we can be less and more super strong!