It is well known that the fragmentation and homogenization of biological samples is a critical first step in the extraction of DNA, RNA and proteins. Who doesn't want to come to a good start? Of course, this is not an easy task. The use of traditional liquid nitrogen grinding is not only time-consuming and laborious, but the effect is not too stable. Especially when dealing with multiple samples, facing the pile of research and development, cross-contamination is also a problem that has to be taken seriously. The world is so big, you should go and see. Today, mechanical sample cracking has become the preferred method of sample preparation in the laboratory because it does not use enzymes, compounds and detergents, and avoids the introduction of downstream inhibitors. At the same time, it is powerful enough to deal with samples that are hard, hard and difficult to handle. MP Bio's FastPrep-24TM 5G sample preparation system is such a powerful instrument. MP Bio has previously introduced several sample preparation instruments, and FastPrep-24TM 5G is the newest member of this family, and 5G represents the fifth generation. Compared to previous products, it uses the touch screen interface for the first time, making operation easier and more intuitive; at the same time, power is enhanced and samples are processed more quickly. With the FastPrep-24TM 5G, you can lyse any hard-to-handle sample in as little as 40 seconds. A new generation, even more powerful Everyone knows that plant seeds are notoriously difficult to do. The seed coat usually contains tannins which inhibit PCR, while the seed material contains starch and fat, making the cleavage difficult. After 40 seconds of cleavage with FastPrep-24TM 5G, the DNA or RNA can be purified using the corresponding kit to obtain high-yield, high-quality nucleic acids without PCR inhibitors, polysaccharides or polyphenols. Organic denaturants or proteases are also not required throughout the process. For today's popular metagenomics research, FastPrep-24TM 5G can also be used in sample preparation. Sample preparation and subsequent separation and purification are very tricky when studying environmental or intestinal samples. In general, soil, sludge, and stool samples are complex, including microbes, plants, animal tissues, and other cells, and may contain PCR inhibitors and degrading enzymes. The 5G instrument can efficiently lyse various microorganisms, including fungal spores and endospores, Gram-positive bacteria and yeast. Downstream combined with a dedicated soil kit, the genomic DNA of these samples can be efficiently separated. At the same time, the FastPrep-24TM 5G is also the first to use the touch screen to control the instrument, which improves the convenience of operation. With a 7-inch high-definition display, you can set parameters with a few taps, simple and intuitive. There are over 70 recommended usage programs in 5G. These programs are validated to include all analytical parameters to help achieve optimal results. For the novice, this is really good news. Of course, you can also save custom programs (up to 12). This system has also added some new features, including the setting of the pause time. During the operation, you can set 1-9 cycles, and each cycle can be set to pause before 1-300 seconds. In this way, after being set up, the whole process can be truly unattended without user intervention. The system comes with a USB interface, which allows you to export the recorded operational data. At the same time, you can also easily upgrade the software through it. In addition, noise is also a problem that cannot be ignored. If the instrument is noisy, it will be tormented to everyone's ears when handling a large number of samples. The FastPrep-24TM 5G has a maximum noise of less than 70 dB and is quieter, allowing you to read the literature while still reading the sample. Correct selection of cracking media This instrument is capable of handling all types of samples, including all types of human, animal and plant tissues; bacterial, yeast and fungal cells, as well as environmental and metagenomic samples. Of course, the samples are different and the lysis medium will be different. To this end, MP Bio has prepared 16 types of lysis media, allowing you to choose from a variety of experiments. how to choose? You should consider factors such as size, shape, and hardness and density. size The smaller the abrasive particles, the smaller the resulting sample particles. The sample particles obtained after grinding have a smaller diameter than the abrasive particles. Therefore, when choosing a lysis medium, consider the size of the sample particles you wish to obtain. For example, if you want a full-featured organelle, it's best to choose a larger lysis medium. Conversely, if it is a smaller structure, such as virions and spores, a smaller lysis medium can be selected. shape The shape is also very particular, it determines the shape of the sample components after cracking. A blunt cleavage medium such as a sphere relies mainly on collision forces to lyse the sample; while a sharp cleavage medium relies primarily on shear forces to lyse the sample. Shear force, like a double-edged sword, is more conducive to breaking difficult samples, but it can also destroy RNA and some easily degraded proteins. Therefore, for DNA, stable proteins, polysaccharide structures, and small molecular substances, shear force is still very practical. Hardness and density The composition of the cracking medium determines two very important parameters: hardness and density. The hardness of the lysis medium must be higher than the hardness of the sample. Therefore, MP Bio's lysis medium can meet the requirements, so density is an important parameter. The higher the density, the better the grinding effect on refractory samples such as spores. In addition, the density of the cleavage media particles needs to be greater than that of the ground solution, otherwise the lysis medium floats on the surface and does not function as a cleavage or homogenization. Having said that, you may have a rough impression, but it is not clear which one to choose. It doesn't matter, please see the picture below, depending on the sample type and application requirements. You can purchase the lysis media tube separately and use your own common buffer system, or you can purchase a complete set of purification kits for best results. There are several sizes of cracking tubes to choose from: 2 mL, 4.5 mL, 15 mL, and 50 mL tubes and 96-well plates. With the different sample counts, sizes and temperatures, the FastPrep-24TM 5G has 11 adapters to choose from. In addition to the five adapters for sample processing at room temperature, there are three adapters for sample processing under freezing conditions, which is a boon for enzyme activity analysis. Once the frozen sample split tube is mounted on the adapter, it can be run directly on the FastPrep-24TM 5G without additional processing. In addition, MP Bio has introduced a new all-metal adapter for highly infectious samples. Since the all-metal adapter can withstand temperatures up to 450oC, it can be sterilized by high temperature and high pressure. In this case, bacteria, viruses, fungi, parasites, viroids, and prions can be eliminated. All all-metal adapters are resistant to detergents and other solvents and are easy to maintain and repair. In conjunction with the use of FastPrep-24TM 5G, MP Bio also offers a variety of purification kits for DNA, RNA and protein extraction. Depending on the type of sample and the substance being extracted, the kit contains the appropriate lysis medium tube, unique buffer and purification system to rapidly and repeatedly lyse and purify the sample. If you have a hard-to-handle sample on hand, try the FastPrep-24TM 5G. You can apply for a free trial now, and seize the opportunity. Bone Glue,Bone Glue Gelatin,Animal Bone Glue,Industrial Grade Bone Glue Hebei Haodong Biological Technology Co.,Ltd. , https://www.hdgelatin.com