Animal tissue genomic DNA rapid extraction kit First, the introduction This product uses a unique dissolution system to quickly prepare DNA from a variety of biological samples for PCR amplification. The method pretreatment of the sample is relatively simple, the extraction process does not need to use toxic phenol chloroform extraction, and no time-consuming alcohol precipitation is required, and the whole extraction process only needs 15-20 min. Second, the composition (48 test) 071051M composition content Buffer A 25ml Buffer B 50ml Centrifuge tube A 1.5ml × 48 Centrifuge tube B 1.5ml × 48 Instruction manual 1 copy After receiving the goods, Buffer A is stored at 2-8 ° C, Buffer B is stored at room temperature (15-25 ° C), and the shelf life is 12 months. Among them, after each use of Buffer A, try to close the cap to reduce the contact with the air. It is normal for Buffer A to appear red or yellow, which can be used normally. 4. Materials and tools that need to be prepared Five, the operation steps 1.1 Sampling requirements For the whole tissue sample, cut the center tissue composition of the sample by 5-10 g; for the mixed sample of the small cut piece, select several small pieces of samples, each of which cuts the center tissue 1-2 g, and mixes the components. For processing samples such as meat rolls, meat slices, meatballs, etc., select several pieces/components and mix the components; for the crushed processed meat foam samples, weigh 1-2 g in five different parts of the sample, mix Each component. 1.2 Homogenization The sample weighed in 1.1 was crushed using a homogenizer or a grinding rod, and 5 g of the crushed sample was transferred to a sterile homogenized bag, and 10 ml of raw was added. The brine is homogenized and mixed. 2.1 Take 20-30 mg of homogenized tissue after homogenization in 1.2, transfer to centrifuge tube A, add 500 μl Buffer A, mix by shaking, and heat bath at 80 °C for 10-15 min. 2.2 After the thermal bath is completed, take 10 μl of the supernatant into the centrifuge tube B, add 1 ml Buffer B for neutralization and mixing, and the neutralized liquid is the DNA solution, which can be used for subsequent experiments. Store at -20 °C. Extremity Pack,Sterile Extremity Pack,Disposable Extremity Pack,Disposable Sterile Extremity Pack Xinxiang Huaxi Sanitary Materials Co., Ltd. , https://www.huaximedical.com
Animal Tissue Genomic DNA Rapid Extraction Kit Instruction Manual
Third, the shelf life