Mouse N-methyl-D-aspartate receptor-1 ( NMDA-R1 ) ELISA kit user's Guide Detection principle The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells previously coated with N-methyl D-aspartate-type receptor antibody (NMDA-Ab), specimens, standards, and HRP-labeled detection antibodies were sequentially added, and the cells were washed and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with N-methyl-D-aspartate receptor-1 (NMDA-R1) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader to calculate the sample concentration. Sample collection, processing and storage methods 1. Serum: Use a tube containing no pyrogen and endotoxin. Avoid any cell irritation during the procedure. After collecting the blood, centrifuge and centrifuge for 10 minutes at 3000 rpm to quickly and carefully separate the serum and red blood cells. 2. Plasma: EDTA, citrate or heparin anticoagulation. The supernatant was taken by centrifugation at 3000 rpm for 30 minutes. 3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymer. 4. Tissue homogenization: The tissue is mashed by adding appropriate amount of physiological saline. The supernatant was taken by centrifugation at 3000 rpm for 10 minutes. 5. Storage: If the sample is not detected in time after collection, please dispense it once, freeze it at -20 °C, avoid repeated freezing and thawing, thaw at room temperature and ensure that the sample is fully thawed evenly. Bring your own items Operational precautions Kit composition name 96-well configuration 48 hole configuration Remarks Microporous ELISA plate 12 holes × 8 12 holes × 4 no Standard 0.3mL*6 tube 0.3mL*6 tube no Sample diluent 6mL 3mL no Detection antibody-HRP 10mL 5mL no 20× washing buffer 25mL 15mL Dilute according to the instructions Substrate A 6mL 3mL no Substrate B 6mL 3mL no Stop solution 6mL 3mL no Sealing film 2 sheets 2 sheets no Instruction manual 1 copy 1 copy no Ziplock bag 1 1 no Note: The concentration of standard (S0-S5) is: 0, 50, 100, 200, 400, 800 pg/mL Reagent preparation Dilution of 20× Wash Buffer: Distilled water was diluted 1:20, ie 1 part of 20× Wash Buffer plus 19 parts of distilled water. Washing method Steps Result judgment Draw a standard curve: In the Excel worksheet, the standard concentration is used as the abscissa, and the OD value is plotted as the ordinate. The linear regression curve of the standard is drawn, and the concentration values ​​of each sample are calculated according to the curve equation. Kit performance Disclaimer
Tof(Time-of-flight), is JRT new product, which is a single-point LiDAR sensor. With a micro size of 46x17x7mm, customers can widely use in many Laser Measurement Solutions. The lidar distance sensor can measure 12m short-range. It's great for Unmanned Aerial Systems.Contact Us Now for data sheet.
ToF Sensor,Time of Flight Sensor,LiDAR Distance Sensor,ToF Distance Sensor Chengdu JRT Meter Technology Co., Ltd , https://www.irdistancesensor.com
Product Name
Tof LiDAR Sensor
Model Number
IT02S-65-A/IT02S-65-B/IT03M-650
Measuring Range (without Reflection)
0.1-12m/0.1-10m/0.1-15m(90% reflectivity)
Place of Origin
China
Accuracy
2cm~4cm/5cm
Blind Area
10cm
Resolution
1cm
Measuring Unit
centimetre
Laser Class
Class II, red
Laser Type
650nm, <1mW
Communication Interface
UART, 115200bps(default)
Size
46*17*7mm/50*26*13mm
Weight
About 4g/10g
Voltage
2.7V~+3.6V
Electrical Level
TTL/CMOS
High Frequency
100hz
Operating Temperature
0-40 ℃ (32-104 ℉ )
Storage Temperature
-25~60 ℃ (-13~140 ℉)