Many malignant tumors that threaten human life have cancer stem cells. In patients with effective tumor treatment, recurrence occurs in the later stage, which is largely related to the ineffective killing of cancer stem cells and their drug resistance. More and more clinical scientists have found that existing tumor treatment drugs may only be effective for tumor cells themselves, but not for cancer stem cells.

In recent years, more and more oncology clinicians and drug research and development companies have begun to develop new drugs for cancer stem cells. But soon many researchers have encountered a problem with very few cancer stem cell resources. Unlike tumor cell lines, tumor stem cells need to be separated from tumor patients' blood or clinically punctured tissue samples, and some samples can only be separated into cells within 10,000. Previously, drug developers were unable to find a sufficiently sensitive protein analysis technique to detect changes in the expression and modification of cancer stem cell proteins treated by new compounds, especially the modification of key proteins. The key protein modification changes can usually reflect whether stem cells respond to new compounds, and it is a key link in drug screening of cancer stem cells.

In January 2015, the two top universities in the UK, the University of Manchester and the University of Glasgow, jointly published their research on the research methods of protein modification in cancer stem cells in the international top journal Nature. Breaking through the bottleneck that cancer stem cells cannot effectively perform protein modification analysis.

Responsible for this research are the University of Manchester Blood Cancer Stem Cell Proteome Research Center and the University of Glasgow Center for Hematology Research.

Two central scientists used flow cytometry to sort CD34+CD38− progenitor cells and CD34+CD38+ directed progenitor cells from patients with chronic myeloid leukemia using dasatinib and imatinib mesylate. The protein was treated with Proteinsimple 's Nanopro 1000 system to detect protein CrkL modification profiles in cell lysates . In the article, the authors point out that Nanopro 1000 cIEF immunoassay technology is a very effective technique for quantifying the percentage of each modified isomer in total CrkL protein with very good repeatability. Subsequent authors continued to use 1.6 ng of total protein to detect pS962 PTPRC/CD45 and PTPRC/CD45, and found that CV was 7.82, 4.41, respectively, and the repeatability was very good.

Nanopro 1000 cIEF immunoassay technology is developed by the American proteinsimple company. Based on capillary isoelectric focusing separation, using original protein capture technology combined with antigen-antibody immuno-hybridization, the protein modification map of trace samples such as stem cells is interpreted in the nano-upgrading reaction system. . The system is fully automated and requires no manual operation, enabling the powerful analysis of 96 samples in 19 hours.

Throat Pain Relief Patch

Medical Cold Patch
Throat Pain Relief Patch
[Name] Medical Cold Patch
[Package Dimension] 36 round pieces
The pain relief patch is composed of three layers, namely, backing lining, middle gel and protective film. It is free from pharmacological, immunological or metabolic ingredients.
[Scope of Application] For cold physiotherapy, closed soft tissue only.
[Indications]
The patches give fast acting pain relief for acute and chronic tonsillitis.
[How To Use a Patch]
Please follow the Schematic Diagram. One piece, one time.
The curing effect of each piece can last for 6-8 hours.
[Attention]
Do not apply the patch on the problematic skin, such as wounds, eczema, dermatitis,or in the eyes. People allergic to herbs and the pregnant are advised not to use the medication. If swelling or irritation occurs, please stop using and if any of these effects persist or worsen.notify your doctor or pharmacist promptly. Children using the patch must be supervised by adults.
[Storage Conditions] Store below 30c in a dry place away from heat and direct sunlight.