Agrocybe aegerita is a kind of wood rot fungus, which has delicious taste, rich nutrition, and good health care function. It is one of the rare edible fungus. Its cultivation techniques are as follows:

I. Cultivation environment conditions

Agrocybe aegerita mycelial growth temperature is 3 °C -35 °C, the most suitable growth temperature is 23 °C -28 °C. At temperatures above 30°C, mycelial growth weakened or even stopped. The developmental temperature for fruit body differentiation is 23°C-26°C, and the optimum temperature is 18°C-24°C. Higher or lower temperatures will delay primordial differentiation. The best fruiting temperature is about 20°C, about 10 days from budding to maturity. Agrocybe during the growth phase of mycelia, the suitable moisture content of the culture medium is 60%-65%, and the relative humidity of the mycelial growth is 65%-70%. Mycelium growth stage does not require light, excessively bright scattered light inhibit mycelial growth. Agrocybe aegypti is aerobic and very sensitive to carbon dioxide. Poor ventilation and high carbon dioxide concentration can cause slender stipe, impeded development of the cap, and easy to open umbrella or deformity. The mycelium can grow in the pH range of 4-7, grow best at pH 4.5-5.5, and the mycelium will grow poorly when the pH is lower than 4 or higher than 7.

Second, prepare before planting

Agrocybe aegerita has an indoor shelf erecting bag, mushrooming, bagging, earthen mushrooming, and wall-bag cultivation. Spring planting is generally carried out in late February to early March. Inoculation takes place in April and May, and it is cultivated in April and May. In autumn, it is planted at the end of August and early September, and in October-November, it is cultivated. Agrocybe saccharomycea bags usually grow in the shed. Choose a simple mushroom house with high ground, sunny ventilation, convenient transportation, near water source, and no pollution around. The structure is best for brick and tile structure. A bed frame can be set in a mushroom house or a mushroom house. Commonly used formulas for culture materials are: 40% of wood chips, 30% of cottonseed husks, 16% of wheat bran, 8% of corn flour, 4% of rapeseed cake powder, 1% of gypsum, 0.5% of sugar, 0.4% of potassium dihydrogen phosphate, and sulfuric acid Magnesium 0.1%.

Third, the bacteria bag production

Weigh the ingredients as usual, adjust the moisture content to about 60%, and the ratio of material to water is about 1:1.2. After the moisture penetrates evenly and in the fashion bag. Inflated bag cultivation is usually carried out in a low-pressure polyethylene bag with a size of 15 cm, 55 cm and a thickness of 0.04 mm. Each bag contains 700 g of dry material. The specific operation of high temperature sterilization is as follows: open the valve heating, wait until the pressure rises to 0.8-1.1 kg/cm2, discharge gas temperature reaches 107°C, control 2-3 hours, ensure that the heat inside the pot has enough time to penetrate Bags. The temperature inside and outside the bag is uniform and then warmed to increase the pressure to 1.5-1.7 kg/cm2 for 105-120 minutes. To burn the fire, it is required that the center temperature of the bag reaches 97°C-100°C within 2 hours after ignition, and then it is maintained for 15-18 hours. There can be no cease-fire on the way, no cooling, no fire, and 100°C. The cultivated original species and cultivars were thick and thick, and were inoculated at both ends of the bag when the material was cooled to 28°C. It is also possible to perform punching and inoculation after sterilization. After inoculation, inoculate the wells with clear tape. Each bottle of bacteria can inoculate 25 bags.

IV. Fungal management

In the first 3 days after inoculation, room temperature was adjusted to 25°C-27°C to make mycelia grow robustly. When the mycelium grows to 1/2 of the material depth, its growth begins to slow down. At this time, loosen the mouth of the bag to loosen the cord, or puncture 1-2 lines of microporous oxygen in the 1-1.5 cm in front of the growth of the hyphae. The relative humidity of indoor air during germination phase should be controlled at about 70%, and there should not be excessive bright light, and doors and windows should be shielded from light.

V. Prompt Bud Management

Outdoor mushroom shed method is as follows: the site is organized into a 30 cm wide, 15 cm high boring machine, the bed is covered with a layer of sand, and then two layers of film, the bag ends of the bag cut open stacked on a trampoline. The direction of stacking of the bacterial bags should be consistent with the direction of the doors and windows of the mushroom sheds, and cutting bags and bags should be carried out at the same time. Prior to the opening of the bacterium bag, 34% carbolic acid solution or deltamethrin 3000 times solution was used to disinfect the bag and disinfested the site. Use a sharp knife to cut the bag's membrane along the tie. After cutting the bag, with the prolongation of the browning time of the mycelia and the deepening of the color, a layer of brown bacilli is formed on the surface around the bag mouth. The normal bacteriostasis was tan and rust brown, shiny, and normal fruiting. Combining with the turning of the bag, the temperature difference is increased for 3-7 consecutive days so that the temperature difference between day and night can reach 8°C-10°C, until many white granules appear and differentiate into mushroom buds.

Sixth, mushroom management

During the mushrooming period, the temperature of the mushroom shed is maintained at 15°C-26°C, preferably 20°C-24°C. After the formation of the primordium, the number of water sprays should be increased to maintain the relative humidity of the air at 85%-90%. In the early stage, water is mainly sprayed into the space and the ground. In the middle and later stages, water can be sprayed directly onto the fruit body. During the growth of the fruiting body, the respiration is strong. During the mushrooming period, the mushroom shed must be ventilated to keep the air fresh. The light intensity at the time of fruiting is maintained at 500-1000 lux. Do not move the bacteria bag at will during the growth period and do not disturb the light source of the mushroom shed. Agrocybe can be used to collect 3-4 mushroom. After each collection of 1 tidal mushroom, the bag is covered with a plastic film, water is stopped for 7-10 days, and the relative humidity of the air is maintained at 70%-75%. After the wounds are collected at the mushroom collection site, the budding is performed again according to the above method.

VII. Harvesting and preservation

When the color of the cap changed from dark reddish-brown to light-colored, and the pellicle membrane had not yet ruptured, it was suitable for harvesting. When harvesting, it is required that whole stalks or single plants be harvested at one time and then the feet of the mushroom be smashed. Fresh mushrooms are dried in air-conditioned rooms at 15°C to 20°C and dried to 80% dry. Then they are packed in plastic bags or foam plastic boxes. Covered in bags or boxes with 4-6 layers of absorbent toilet paper, sealed and refrigerated. The storage temperature is 5°C-10°C. During transport, the mushroom body is properly compacted to prevent vibration in the packaging container.

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