(1) Materials Agar slant medium The cultured plate culture was isolated. (2) Method 1 Mark the name (or number) of the transplanted seed, the date of inoculation, the group of the inoculator, and the code on the upper part of the agar slant medium test tube. 2 Hold the bottom of the plate with bacteria on the left and hold the inoculation ring on the right. After the inoculation loop was sterilized and cooled on the flame, the well-developed isolated colonies on the plate were drawn. Put the plate back in place, immediately use this hand to take the inclined medium to hold it diagonally, hold the cotton plug with the little finger of the right hand and the palm of the hand, gently rotate and then pull out the cotton plug, and the nozzle is sterilized by flame. 3 Extend the inoculated loop of the adherent bacteria into the coagulated water at the bottom of the tube of the slant medium, draw a straight line from the bottom along the surface of the slant medium, and then draw a continuous zigzag line from the bottom; or bend the line without a straight line. . Take out the inoculating loop, sterilize the tube by flame, plug the tampon, put it on the tube rack, and sterilize it by inoculation ring flame. If the bacteria transferred to the slant medium are grown on the slant medium or are grown in the liquid medium, the left hand is simultaneously slanted with the tube and the slant medium, and the right hand ring finger and the little finger The palm is simultaneously pulled up to hold the two tampon. After the tube mouth and the inoculating ring are sterilized by flame, the bacteria are taken from the strain tube and immediately transplanted to the inclined surface, the cotton plug is stuffed, and the ring flame is sterilized and put down. The slant medium was then incubated for 24 h in a 37 ° C incubator. As a result, the surface of the slope formed a lawn. Frozen Seafood Mix,Mixed Seafood Bags,Frozen Seafood Mix Bags,Frozen Seafood Cocktail Zhoushan Haiwang Seafood Co., Ltd. , https://www.haiwangseafoods.com