The CRISPR/Cas9 system is a very efficient method of gene editing, but most of the animals that have been genetically edited have chimerism, meaning that only a subset of their genes have been edited. For studies involving phenotypes, chimeric genetically edited animals require further cross-breeding to obtain complete knockout animals. When it comes to large animals, such as non-human primates, the problem of chimerism is particularly serious because macaques have a reproductive cycle of 5-6 years and only one cub per litter. Many researchers have tried to produce genetically modified animals without chimerism, especially large animals. This involves injecting Cas9 mRNA and sgRNA into oocytes rather than fertilized eggs, or injecting Cas9 protein, or injecting dual sgRNA. However, after DNA sequencing analysis of individual animal tissues of these methods, it was found that these methods are quite inefficient in performing biallelic knockout. On June 6th, Cell Research published an online report on the use of improved "C-CRISPR" technology to obtain mice and monkeys with complete knockout of single or multiple genes. By injecting multiple continuous guide RNAs (sgRNAs) against gene exons into fertilized eggs, the study allows high-throughput production of various gene-knockout mice for phenotypic analysis and rapid production of knockout monkeys. model. The research was carried out by the Yang Hui Research Group of the Institute of Neuroscience of the Chinese Academy of Sciences, the Center for Excellence in Brain Science and Intelligent Technology, the Xiong Zhiqi Research Group, and the Sun Qiang Research Group of the Institute of Non-human Primate in Suzhou. In the study, the researchers found that a cocktail mixture of Cas9 mRNA and multiple sgRNAs into fertilized eggs, 100% complete deletion of single or multiple genes in mouse embryos, and 91%-100 in monkey embryos % removed. This method can simultaneously generate insertion deletion and exon deletion, thereby achieving efficient gene complete knockout. The researchers also used the C-C CRISPR method to establish F0 mice with completely knocked out gene functions for rapid phenotypic analysis of gene function and F0 monkeys with complete knockout of gene function. Finally, high-throughput sequencing analysis revealed that C-CRISPR did not introduce significant off-target changes in genetically edited mice and monkeys. Iridology Camera Health Analyzer Iris Iriscope Iridology Camera:
Iris holographic diagnostics is a kind of knowledge that applies non-drug and natural therapy to analyze and care for the body by analyzing the iris and pupil to analyze and regulate the health condition of the body. In the field of health medicine, it is called non-invasive health testing and non-drug health intervention. It is a traditional clinical health medicine in Europe.
Iris Iriscope Iridology Camera System Functions:
Iridology Camera,Usb Iridology Camera,Iriscope Iridology Camera,Iridology Camera Eye Iriscope Shenzhen Guangyang Zhongkang Technology Co., Ltd. , https://www.syztreatment.com
1. Adjust brightness through either software or switch on handle line, delete the photos, and adjust the focus, quick fixed photos with white balance and high stability of colors. Connects directly with computer without outside power and easy to operate.
2. The software can save the client information and irises also the details of products that you recommend them. After taken the photos, you can analysis the irises and later compare the irises pictures when your client comes back to see their progress. Can print an analysis report. Save the photos according to the date & time you take the photos.3. This machine will help the client know his health condition, including the problem which you have had in the past. The Iridology will be your health counselor, tell you how to keep away from the illness.
Iris Iriscope Iridology Camera Specification:
* Nice appearance and innovative design
* LED illuminator around lens
* Imported lens with plated layer
* 12MP high resolution CCD sensor
* Special DSP image processor, Optical Image Stabilizer
* Single capture button and digital pause capture.
* Adjustable focus to give clear image.
* Auto white balance and contrast adjustment, Color Temperature Filter
* Compatible with iris lens, hair lens.
* Deliver clear and accurate images.
* Easy to operate.
* OS: Windows XP, WIN2000, 2003, Vista, Win7. Win8,win 10
What is the primary benefit of photo iridology?
Not only does it bring clues to your iridology analysis, but it helps to create a better connection with your patient. The patient, who`s naturally curious, is eager to see their own iris. With some simple explanations they can get a basic understanding of your analysis. The connection with your patient strengthens and their understanding of their process improves.
* Iris analysis system: international technology, unique functions.
* Iris analysis system is a medicinal tool that checks the body conditions and prevents diseases from occurring.
* We brought in the advanced iris analysis technology from Germany to lead people to discover sources of illness, and care the body health and spirit in anyways.
* The instrument can show the body conditions of customers and suggest customers the suitable health food, and the plans to care their bodies.