In the past few weeks, Han Chunyu, an associate professor at Hebei University of Science and Technology, has once again become a hot spot in the biological academic circle. First, Han Chunyu submitted new data (Editor's note: non-original data). On the morning of January 19th, "Nature-Biotechnology" issued a statement that will be investigated and then made the next decision. Then, on the afternoon of the same day, Hebei University of Science and Technology announced that it had signed a cooperation agreement with Novozymes on NgAgo. Novozymes said that it explored the role of NgAgo in the production of enzyme microbial expression systems. In the academic circle, on January 20th, the team of Jin-Soo Kim, the genetic editing center of the Korea Institute of Basic Science, published a new NgAgo paper on the pre-print website, saying that NgAgo could not cut DNA and only cut RNA. Some readers raised the question: Is there a “reversal†in the controversy surrounding the Han Chunyu NgAgo paper behind the intensive news dynamics? Associate Professor of Hebei University of Science and Technology Han Chunyu Picture from the network Before making a judgment, there are four focus issues that need to be clarified. The author combs as follows: Focus 1: What is the question about Han Chunyu's NgAgo paper? On May 2, 2016, Han Chunyu's research group published a paper on NgAgo technology in Nature-Biotechnology. Since July last year, doubts about the inability of the NgAgo experiment to be repeated have begun to culminate with the questionable review articles published in Proteins and Cells and Nature-Biotechnology. To this day, "the experiment can not be repeated" is still on the way to NgAgo as a "genetic scissors." The questioning of NgAgo technology is limited to a specific scope, and the NgAgo paper published by Han Chunyu is delineated. In the paper, Han Chunyu reported that he used NgAgo technology in the mammalian cell system to use DNA as a guide to efficiently cut DNA double strands. "Lactating cells", "efficient", and "cutting DNA" are all essential keywords for Han Chunyu's NgAgo paper. Without these keywords, it is difficult for NgAgo to board the international journal Nature-Biotechnology with more than 40 impact factors. Without these keywords, NgAgo will not attract the global laboratory to test it. The reason for this is that Ago protease has the potential to be genetically edited as early as 2014 by the Dutch scientist John van der Oost, except that most of the enzymes in the Ago family need to be above 60 degrees Celsius. It is active, which means that it is not practical in mammalian cells and cannot be used by humans. Furthermore, the third-generation gene editing technology, CRISPR, performed well in the laboratory. If a technology is not as efficient as CRISPR, this technology cannot make a breakthrough. Summary: The questioning of Han Chunyu's NgAgo paper is that according to the experimental operation in the paper, efficient gene editing in mammalian cells cannot be achieved. A Foot Spa Machine with heat is a device used to provide a relaxing and therapeutic foot massage. It usually has a basin filled with warm water and has various massage settings such as vibration, bubbles, and rollers. The heat function helps soothe tired and aching feet, while the massage setting provides a deep-tissue massage that helps improve circulation, reduce tension, and relieve pain. Some foot spas also come with removable attachments, such as pumice stones and brushes, for extra exfoliation and cleansing. Overall, a foot spa machine with heat is a great tool for anyone looking to pamper their feet and promote overall relaxation and wellness. Foot Spa Machine With Heat,Bubble Foot Bath Massager,Foot Massage Machine,Pedicure Foot Spa Machine Huaian Mimir Electric Appliance Co., LTD , https://www.mimirfootbath.com