Anaerobic bacteria cannot grow under aerobic conditions. To cultivate anaerobic bacteria, an anaerobic environment must be created. The reducing agent is usually added to the medium, or the free oxygen in the environment is physically and chemically removed to reduce the redox potential. Such as blister medium, sodium thioacetate medium, bovine heart brain infusion medium and the like. There are many commonly used anaerobic culture methods, which can be selected according to actual conditions. 1. Anaerobic cylinder method. The plate or liquid medium test tube inoculated with the specimen can be placed in an anaerobic tank. The anaerobic tank is an ordinary drying cylinder, and the anaerobic environment is caused by physical and chemical methods to culture the anaerobic bacteria. 2. The anaerobic bag (Bio-bag) creates an anaerobic environment in an anaerobic environment in a plastic bag. The plastic bag is transparent and non-breathable, and contains a gas generating tube (sodium bicarbonate solid sodium bicarbonate and 5% citric acid ampoules), a Meilan indicator tube, a palladium catalyst tube, and a desiccant. After placing the inoculated plate, squeeze out the air in the bag and seal the bag. First break the gas generating tube, then break the Meilan indicator tube, and the inside of the bag will cause an airless environment within half an hour. If no mutation indicates that the bag has reached an anaerobic state, it can be incubated. 3. The Anaerobie glove box is one of the best internationally recognized instruments for anaerobic culture. It is a sealed large metal box with a transparent glass plexiglass front panel and two gloves on the board that can be operated in the box by gloves, hence the name. There is an exchange room on the side of the box, which has two inner and outer doors, and the inner door is closed inside the box. To put the object into the box, first open the outer door, put it into the exchange room, close the outer door for pumping and ventilation (H2, CO2, N2) to reach the anaerobic state, then hand into the glove to open the exchange room door, and put the item Move into the box and close the inner door. The anaerobic state is maintained in the tank, and the principle of utilizing hydrogen in the aeration to oxidize the water under the catalysis of palladium and in the tank is oxidized. The box can adjust the temperature. It is attached to the incubator or incubator. It can also be placed in a dissecting microscope to observe the anaerobic bacteria colonies. This anaerobic tank is suitable for large-scale cultivation of anaerobic bacteria. The base can be placed in a pre-reduction and anaerobic sterility test. The suction, aeration, anaerobic environment and temperature of the metal hard wall type anaerobic tank are automatically adjusted. 4. Anaerobic box: The principle is the same as anaerobic bag, and there is finished product sales. 5. Biological oxygen consumption method: put a living organism (mostly a plant) in a closed container, consume oxygen, and simultaneously produce carbon dioxide for bacterial growth. 6. Pyrophoric acid method: Spread a gauze or filter paper on a clean glass slide, evenly spread the charred acid acid, and then mix it with NaHCO3 powder or NaOH solution to quickly reverse the plate that has been inoculated with bacteria. On top, it is sealed with melted white wax to create a closed space. The pyrophoric acid reacts with the base to consume oxygen. This method is simple for the cultivation of anaerobic bacteria with anaerobic rigor. If there is Clostridium. 7. Blister culture medium: It is an anaerobic culture method that does not require special equipment. The blister and broth are placed in a large test tube, and the liquid surface is sealed with Vaseline, resulting in an oxygen-free environment. 1 thread anaerobic culture tube CLS-4208 series 16 x 125 mm threaded tube anaerobic culture conditions. Flanged locking device made of butyl rubber. The complete kit consists of a disposable glass tube, a stopper and a 9 mm open nut. The package specification is PACK OF100. 2 aluminum seal anaerobic culture tube CLS-4209 series An improved version of the threaded anaerobic tube. Redesign the sealing structure to maximize the seal between the nozzle and the plug and improve the tightness of the hermetic seal. The specially formulated blue chlorobutyl rubber stopper provides greater sealing and supports repeated puncture of the syringe. The newly designed aluminum seal avoids the risk that the screw cap may not be closed. The disposable glass tube is 18 x 150 mm and is made of Type I and Class B borosilicate glass. The complete package includes disposable glass tubes, blue chlorinated butyl rubber stoppers and aluminum seals. The package specification is PACK OF100. 3 anaerobic culture bottles Under anaerobic conditions, anaerobic bottles are designed for research involving methanogenic bacteria, cell culture, drug discovery, research and development. This bottle is made of Duran® Schott borosilicate glass. The bottle is sterilizable at high temperatures. Each bottle has a scale indicating an approximate volume and a writing area for identification. The complete kit includes a bottle, rubber stopper and aluminum seal. Note: Plastic coated bottles are available. Shanghai Shujun Instrument Equipment Co., Ltd. | Ji Xuedong Salinomycin Anticoccidial,Salinomycin Veterinary Use,Salinomycin Feed Additives,Salinomycin Animal Medicine Shandong Shengli Bioengineering Co., Ltd , https://www.shenglipharm.com
Shanghai Shujun Instrument Equipment Co., Ltd. provides anaerobic culture solutions for the anaerobic culture series of Chemglass brand imported from the United States: 
