Microvascular and nerve water separation methods (taking the arteriovenous nerve water separation as an example)

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Microvascular and nerve water separation methods (taking the arteriovenous nerve water separation as an example)

Femoral arteriovenous nerve water separation

The separation of tiny arteries, veins, and nerves within 1 mm. This separation method utilizes the expansion of connective tissue with a high water content between the arteries and veins and the nerves to achieve the purpose of blunt separation.

This method is simple and easy, and is a commonly used method for microvascular vascular separation in experimental animals.

Huaifeng poetry x7E32G needle, 1ml syringe, saline

Huaifeng è­¥:

"Huaifeng" poetry, the lower limbs are placed on the back.

2, cut the inguinal skin, bluntly stripped part of the inguinal fat, fully exposed femoral arteries and nerves.

3. Under the microscope, the connective tissue between the arteries and veins is bluntly torn open.

4. Connect the prepared prefilled saline syringe 30G needle. The needle needs to be passivated.

5. Insert the passivated 30G needle into the appeal mouth and push the saline edge along the arteriovenous direction to extend forward to reach the length required for the experiment.

6. At this time, the connective tissue between the femoral artery and the nerve will form a long strip of water, which will naturally separate the arteries and veins and nerves.

7. The sharp scorpion tears the water bag formed by the connective tissue, and the arteries and veins and nerves are separated. Carefully clean the connective tissue on the surface.

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