Transportation conditions : normal temperature transportation

Item: 51021: 50 reactions

51022: 100 reactions

Features

â—ŽThe operation is simple and fast, and the operation can be completed within half an hour;

â—ŽHigh purity of extracted RNA, no inhibitor, A260/A280 is 1.8-2.0; â—Ž high yield, more RNA extracted by the same sample size;

â—ŽThe specimen has a wide range and is suitable for cell discrete specimens such as cultured cells, acupuncture extracts, and urine sediment cells;

â—Ž It is free of toxic solvents such as phenol and chloroform, safe and non-toxic.

Kit composition

product description

The BIOG RNA Cell Kit is a kit developed by our company to extract genomic RNA from various cell samples. The sample range extracted includes discrete samples of cells such as cultured cells, bacteria, acupuncture extracts, and urine sediment cells. The BIOG RNA Cell Kit uses a complex digestive juice to digest the proteins in the cells, making the cellular RNA easier to release. Therefore, the RNA extraction yield of this kit is high. The BIOG RNA Cell Kit uses the latest ion-exchange membrane technology and has repeatedly optimized the lysate and eluent formulations. The extracted RNA has higher purity and maximizes the removal of impurities such as proteins, pigments, lipids, etc., and can be directly applied. Various conventional molecular biology experiments such as Northern hybridization, RT-PCR, Real Time RT-PCR, cDNA library construction, and in vitro translation.

Steps:

1. Please prepare yourself: absolute ethanol, PBS and 1.5mL centrifuge tube without RNase.

2. Remove the precipitate and wash solution as follows:

a) Precipitate: 4.5 mL was added to 25.5 mL of absolute ethanol; 9 mL was added to 51 mL of absolute ethanol.

b) Washing solution: 9 mL of 21 mL of absolute ethanol was added; 18 mL of 42 mL of absolute ethanol was added.

c) If the prepared precipitate is precipitated, it can be dissolved at 37 ° C, shaken and used.

3. Cell treatment: a) culture the plate cultured single-layer adherent cells, discard the culture medium, wash once with PBS, discard PBS;

b) Cultured cultured adherent cells should be trypsinized, treated as cell suspension, centrifuged at 1,000 rpm for 5 minutes, the supernatant is completely discarded, and 100 μL of PBS is added to shake the cells to suspension;

c) The cultured cells were suspension cells, centrifuged at 1,000 rpm for 5 minutes, the supernatant was completely discarded, and 100 μL of PBS was added thereto, and the cells were suspended.

4. Add 200 μL of lysate, 20 μL of the digestive juice, mix well by shaking, and centrifuge at 56 ° C for 10 minutes until the cells are completely lysed.

5. Add 500 μL of the precipitate, gently mix by inversion, if there is a semi-transparent suspension, does not affect the extraction of RNA and subsequent experiments.

6. Place the adsorption column into the collection tube, transfer the solution into the adsorption column, let stand for 2 minutes, centrifuge at 12,000 rpm for 4 minutes at 4 ° C, and discard the waste liquid in the collection tube.

7. Place the adsorption column back into the collection tube, add 500 μL of the washing solution to the adsorption column, centrifuge at 12,000 rpm for 4 minutes at 4 ° C, and discard the waste liquid in the collection tube.

8. Place the column back into the collection tube and centrifuge at 12,000 rpm for 4 minutes at 4 ° C to remove any remaining wash solution.

9. Remove the adsorption column, place it in a new RNase-free 1.5 mL centrifuge tube, add 50-200 μL of eluate, let stand for 3 minutes, centrifuge at 12,000 rpm for 4 minutes at 4 °C, and collect the RNA solution.

Store at 10.-70 ° C or directly in the next experiment.

Precautions

1. To prevent RNase contamination, it is best to wear disposable clean gloves, a mask, a treated RNase-free container and RNase-free ultrapure water during the experiment.
2. Both the precipitate and the washing solution contain irritating chemicals. Please take protective measures to avoid direct contact with the skin and prevent inhalation of the nose and mouth. If you accidentally get into skin or eyes, rinse immediately with water or saline. Seek medical attention if necessary.
3. The amount of cells is recommended to be no higher than 10 ⁷ .
4. It is normal for the lysate to precipitate as a white floc, and it can be dissolved in a 37 ° C water bath.

Storage, transportation and expiration date

The kit can be transported at room temperature, stored at room temperature (15-25 °C), valid for 12 months, and stored in digester at -20 °C to avoid repeated freezing and thawing.

QC

This product has passed strict quality inspection and proved that there is no biological pollution.

Note This product is only for scientific research experiments.

Changzhou Baidai Biotechnology Co., Ltd.

Address: New Oriental Logistics Park, No. 51 Dongfang East Road, Changzhou Tel: 0519-88050392 Fax: 0519-83382790