The full name of EMSA is Electrophoretic Mobility Shift Assay. Chinese is called gel migration experiment or electrophoretic mobility experiment. It is a common technique for studying the interaction between DNA and protein or RNA and protein, and can be used for qualitative and quantitative analysis. The following figure shows the basic principle of EMSA: Related Technical Services: Electrophoretic Mobility Experiment (EMSA) Service Loratinib Intermediates Cas 1454847-96-1 Loratinib Intermediate,Loratinib Intermediate,Cas 914225-70-0 Loratinib Intermediate,5-Fluoro-2-Iodoacetophenone Shijiazhuang Dingmin pharmaceutical Sciences Co.,Ltd , https://www.dingminpharma.com Schematic diagram of EMSA experimental principle
This technique is based on the principle that DNA/protein or RNA/protein complexes have different mobility in polyacrylamide gel electrophoresis (PAGE). First, the protein is bound to the end-labeled nucleic acid probe, and then the PAGE gel is used, and the protein-bound complex is slower than the unbound protein probe, so that it can be judged from the electrophoresis result of the PAGE gel. Whether the protein binds to a particular sequence or whether the sequence binds to a protein. (This schematic source is Hundred Bios Mall)