Liaoning Pancake,Mini Pancakes,Mousse And Pancake,Manual Pancake Zhejiang Shanying Trading Co.,Ltd. , https://www.shanyingtrading.com
TL series animal tissue grinder animal tissue grinding sample typical experimental method
TL series animal tissue grinder
Typical experimental method for animal tissue grinding samples
The TL series animal tissue grinder has a pair of symmetrical pair of high-speed and large-amplitude rocker arms. It can easily grind, pulverize, mix and break cells in a few seconds to minutes by irregularly impacting and rubbing back and forth in the sample tube. wall. Fine grinding, the finest can reach 5 microns.
For different samples, the TL series of animal tissue grinders need to be treated under different conditions, mainly related to the grinding speed, grinding time, grinding temperature and the choice of grinding beads. The following is an experimental method for plant samples from the TL series multi-sample tissue grinder, for reference only.
Insects animal experiments a tissue grinder
Experimental Site: Key Laboratory of Silkworm Genomics, Southwest University
Experimental Materials: Silkworm moth, silkworm cocoon tissue
1 Take a silkworm moth, cut it, and take a piece of body tissue (no more than 8mm in diameter) in a 2ml centrifuge tube (EP tube).
2 Add 1 5mm tungsten carbide grinding beads to the tube
3 Add the sample and the EP tube of the beads, place it on the adapter, open the lid, and add liquid nitrogen to freeze; after 3~5 minutes, remove the moisture from the sample and freeze the lid of the EP tube in time. Continue to cool in liquid nitrogen for 3 minutes.
4 Quickly place the adapter on the TL Series Animal Tissue Mill .
Milling at 1600 rpm for 2 minutes using a TL series animal tissue grinder provides a finely divided sample powder for nucleic acid or protein extraction. (grinding time and speed can be further optimized)
The same method is feasible for silkworm cocoons, the grinding time can be increased appropriately, and the effect is very good!
Similar subjects:
Using insects as research materials; experiments requiring high-throughput extraction of nucleic acids and proteins for genetic or physiological and biochemical analysis
Experiment two mammalian tissue grinder
Experimental site: Key Laboratory of Biotherapy, West China University of Medical Sciences
Experimental Sample: mouse heart, spleen, liver (animal offal tissue nucleic acid extraction)
1. The mouse tissue was frozen at -80 ° C, and the diameter was not more than 5 mm. It was placed in a 2 ml round bottom centrifuge tube.
2. Add the protease inhibitor lysate to the centrifuge tube; after 3 to 5 minutes, the tissue block is thawed in the lysate.
3, add 5mm stainless steel grinding beads 1-2
4, using TL series animal tissue grinder 1500rpm, grinding 2min
The results showed that the three tissues were basically broken, the liver tissue was lysed more thoroughly, and the heart and spleen may see fragments (such as better effect with fresh tissue), which can be solved by repeated grinding.
Similar subjects:
Samples were treated with mammalian visceral tissue; the sample volume was small and the flux was high. Experiments are needed to extract nucleic acid material from visceral tissue or to analyze physical and chemical components.
TL series animal tissue grinder is a veritable laboratory sample preparation versatile, which can quickly pulverize and homogenize hard, soft and elastic samples, meet the requirements of physical and chemical analysis laboratories, and configure different volumes and different Grinding tanks for materials can be dry-grinded, wet-grinded, and freeze-ground. They can also be used for cell disruption and DNA/RNA extraction. They are widely used in biomedicine, agriculture, food, and testing.