Green coffee bean extract is made from the green beans of the coffea Arabica plant. There are two types of coffee plants, arabica and robusta. The arabica is higher in quality and higher in chlorogenic and caffeic acids, two primary compounds responsible for anti-oxidant activity. Coffee might have anti-cancer properties, and researchers found that coffee drinkers were 50% less likely to get liver cancer than nondrinkers.
Product features:
1. Special large package for industrial raw material sales(10kg/20kg);
2. 100% pure coffee;
3. Good instant solubility;
4. Stable raw material origin and long-term supply
Functions:
Losing weight.
Green Coffee Bean Powder,Green Coffee Powder Price,Green Coffee Powder Online,Green Coffee Bean Extract Powder Yunnan New Biology Culture Co,.Ltd , https://www.lvsancoffee.com
Anti-virus; Anti-bacteria; Anti-cancer; Anti-aging; Anti-infectious.
Lowering toxicity.
Lowering blood pressure.
Reducing the risk of diabetes.
Help with muscle fatigue for athletes and bodybuilders.
Green coffee bean has strong anti-oxidant properties similar to other natural anti-oxidants like green tea and grape seed extract. Green Coffee Beans have polyphenols which act to help reduce free oxygen radicals in the body. Green coffee bean extract is sometimes standardized to more than 50% Chlorogenic Acid. Chlorogenic Acid is the compound present in coffee which has been long known as for its beneficial properties. This active ingredient makes green coffee bean an excellent agent to absorb free oxygen radicals; As well as helping to avert hydroxyl radicals, both which contribute to degradation of cells in the body.
Rat Interleukin-6 (IL-6) ELISA Kit Instructions for Use
Shanghai Xitang Biotechnology Co., Ltd. 021-55229872, 65333639
Rat Interleukin- 6 (IL-6) ELISA Kit Instructions for Use
 ( used in serum, plasma, cell culture supernatants and other biological fluids )
principle
This experiment used double antibody sandwich ABC-ELISA. The anti-rat IL-6 monoclonal antibody was coated on the microtiter plate, and the IL-6 of the standard and the sample was combined with the monoclonal antibody, and biotinylated anti-rat IL-6 was added to form an immune complex. On the horseradish peroxidase-labeled Streptavidin combined with biotin, the substrate working solution is blue, and finally the stop solution sulfuric acid is added, and the OD value is measured at 450 nm. The IL-6 concentration is proportional to the OD value, which can be passed. A standard curve was drawn to determine the IL-6 concentration in the specimen.
Kit composition ( 2-8 ° C preservation)
Coated Wells
96 holes
Enzyme Conjugate
12ml
10× specimen dilution (Sample Buffer)
12ml
20×Wash Buffer
50ml
Standards: 10ng/bottle
2 bottles
Substrate working fluid (TMB Solution)
12ml
Primary antibody working solution (Biotinylated Antibody)
12ml
Stop Solution
12ml
Prepare reagents and collect blood samples
1. Collection of specimens: serum, plasma (EDTA, citrate, heparin anticoagulation), cell culture supernatant, tissue homogenate, etc., as early as possible, stored at 2-8 ° C for 48 hours; longer time must be frozen (-20 ° C Or -70 °C) to avoid repeated freezing and thawing.
2. Standard solution preparation: Add 0.5 ml of distilled water before use and mix to prepare a 20 ng/ml solution. Set the standard tube 8 tube, the first tube plus the standard dilution 900ul, the second to the eighth tube to add the sample dilution 500ul. Add 100 ul of 20 ng/ml standard solution to the first tube, mix and aspirate 500 ul with the sampler, and transfer to the second tube. Repeat the dilution as described above, and remove 500 ul from the seventh tube and discard it. The eighth tube is a blank control.
3. The 10× specimen dilution was diluted 1:10 with distilled water (example: 1 ml concentrated dilution + 9 ml distilled water).
4. Washing solution: diluted 1:20 with distilled water (example: 1 ml concentrated washing solution added to 19 ml of distilled water)
Test procedure
1. Loading: Add 100 ul of standard or sample to be tested in each well. Mix the reaction plate thoroughly and let it stand at 37 °C for 120 minutes.
2. Wash the plate: Wash the plate thoroughly with washing solution 4-6 times, and dry it on the filter paper.
3. Add 100 ul of the first antibody working solution to each well. The reaction plate was thoroughly mixed and placed at 37 ° C for 60 minutes.
4. Wash the board: the same as before.
5. Add 100 ul of enzyme-labeled antibody working solution per well. The reaction plate was placed at 37 ° C for 30 minutes.
6. Wash the board: same as before.
7. Add 100 ul of substrate working solution to each well and let it react at 37 ° C for 15 minutes in the dark.
8. Add 100 ul of stop solution to each well and mix.
9. Measure the absorbance at 450 nm using a microplate reader within 30 minutes.
Result calculation and judgment
1. All OD values ​​should be subtracted from the blank value before calculation.
2. Take the standard products 2000, 1000, 500, 250, 125, 62.5, 31.2, 0 pg/ml as the abscissa and OD as the ordinate. Draw on the coordinate paper and draw the standard curve.
3. Find the corresponding IL-6 content on the graph based on the OD value of the sample.
Kit performance
1. Sensitivity: The minimum IL-6 detection concentration is less than 16pg/ml.
2. Specificity: Recombinant or natural rat IL-6 can be detected simultaneously. Does not cross-react with other cytokines in rats.
3. Repeatability: The coefficient of variation in both the plate and the plate is less than 10%.
Precautions
1. It is recommended to make double holes for the above standard holes and samples to be tested. The standard curve should be made at the same time for each measurement.
2. The washing process is critical. Insufficient washing will result in an accuracy error and an erroneous rise in the OD value.
3. After the slats are opened, the remaining slats should be sealed again to keep the slats dry .
4. This kit should be stored in a 4oC refrigerator.
5. This kit is for scientific research only and cannot be used for clinical diagnosis!